Hemoglobin electrophoresis: he aha ia, pehea e hana ʻia ai a no hea ia

Anter

• No ke aha ia
• Pehea e hana ai
• Pehea e wehewehe ai i nā hopena

ʻO Hemoglobin electrophoresis kahi hana diagnostic e kuhikuhi ana e ʻike i nā ʻano hemoglobin i hiki ke loaʻa ke kaapuni i ke koko. ʻO Hemoglobin a i ʻole Hb kahi protein i loko o nā hunaola ʻulaʻula i kuleana no ka hoʻopaʻa ʻana i ka oxygen, e ʻae ana i ka lawe i nā aʻa. E aʻo hou aʻe e pili ana i ka hemoglobin.

Mai ka ʻike ʻana i ke ʻano o ka hemoglobin, hiki ke nānā inā loaʻa kekahi kanaka i kekahi maʻi e pili ana i ka hemoglobin synthesis, e like me thalassemia a i ʻole sickle cell anemia, no ka laʻana. Eia nō naʻe, e hōʻoia i ke kuhi, pono pono e hana i nā hoʻokolohua hematological a me biokimia ʻē aʻe.

No ke aha ia

Nonoi ʻia ʻo Hemoglobin electrophoresis e ʻike i nā hoʻololi kūlohelohe a me nā hana e pili ana i ka hemoglobin synthes. No laila, hiki ke koi ʻia e ke kauka e ʻike pono i ka maʻi anemia o ka cell sickle, ka maʻi hemoglobin C a me ka thalassemia ʻokoʻa.

Hoʻohui ʻia, hiki ke noi ʻia me ka pahuhopu o ka ʻōlelo aʻo ʻana i nā kāne e makemake ana e hānau keiki, no ka laʻana, ʻike ʻia inā loaʻa kahi manawa e loaʻa i ke keiki kekahi ʻano maʻi maʻi koko e pili ana i ka synthesis o hemoglobin. Hiki ke kauoha ʻia ʻo Hemoglobin electrophoresis ma ke ʻano he hōʻike maʻamau no ka nānā ʻana i nā mea maʻi i hōʻike mua ʻia me nā ʻano hemoglobin.

I ke ʻano o nā pēpē hou, ʻike ʻia ke ʻano hemoglobin ma o ka hoʻāʻo ʻana o ka kuʻekuʻe wāwae, a he mea nui ia no ke kuhi ʻana i ka maʻi anemia o ka maʻi maʻi. E ʻike i nā maʻi hea e ʻike ʻia e ka hoʻāo kuekue wāwae.

Pehea e hana ai

Hana ʻia ka electrogoresis hemoglobin mai ka hōʻiliʻili ʻana o kahi hāpana koko e kekahi loea i hoʻomaʻamaʻa ʻia i kahi keʻena hana kūikawā, no ka mea ʻo ka hōʻiliʻili pololei ʻole e hopena ai i ka hemolysis, ʻo ia hoʻi ka luku ʻana i nā hunaola ʻulaʻula, i hiki ke hoʻopilikia i ka hopena. Hoʻomaopopo i ka hōʻiliʻili ʻana i ke koko.

Pono e hōʻiliʻili ʻia ka hōʻiliʻili me ka mea hoʻomanawanui e hoʻokē ʻai ana ma kahi o 4 mau hola a ʻo ka hāpana i hoʻouna ʻia no ke kālailai ʻana i ke keʻena hoʻokolohua, kahi e ʻike ʻia ai nā ʻano hemoglobin i loaʻa i ka mea maʻi. I kekahi hale hana, ʻaʻole pono e hoʻokē ʻai no ka hōʻiliʻili ʻana. No laila, he mea nui e ʻimi i ke alakaʻi mai ke keʻena hoʻokolohua a me ke kauka e pili ana i ka hoʻokēʻai no ka hōʻike.

Hoʻomaopopo ʻia ke ʻano o ka hemoglobin e electrophoresis i ka alkaline pH (ma kahi o 8.0 - 9.0), kahi ʻano hana e pili ana i ka nui o ka neʻe ʻana o ka mole i ke ʻano o ka uila, me ka ʻike ʻia o nā pēpē e like me ka nui a me ke kaupaona o ka molekoki. Wahi a ke kumu band i loaʻa, hana ʻia kahi hoʻohālikelike me ke kumu maʻamau a, no laila, hana ʻia ka ʻike o nā hemoglobins ʻokoʻa.

Pehea e wehewehe ai i nā hopena

Wahi a ke ʻano o ka bana i hōʻike ʻia, hiki ke ʻike i ke ʻano o ka hemoglobin o ka mea maʻi. ʻOi aku ka kiʻekiʻe o ka mole mole o Hemoglobin A1 (HbA1), ʻaʻole ʻike ʻia ka nui o ka neʻe ʻana, ʻoiai ʻoi aku ka māmā o HbA2, e komo ana i loko o ka gel. Ua unuhi ʻia kēia ʻano band i loko o ke keʻena hoʻokolohua a hoʻokuʻu ʻia i ke ʻano o kahi hōʻike i ke kauka a me ka mea hoʻomanawanui, e hōʻike ana i ke ʻano o ka hemoglobin i loaʻa.

Loaʻa ka fetog hemoglobin (HbF) i nā kiʻekiʻe kiʻekiʻe i loko o ka pēpē, eia nō naʻe, i ka hana ʻia ʻana, hoʻemi nā HbF i ka hoʻonui ʻia o HbA1. No laila, ʻokoʻa nā mea like o kēlā me kēia ʻano hemoglobin e like me ka makahiki, a maʻamau lākou:

Eia nō naʻe, loaʻa i kekahi poʻe i nā hoʻololi kūlohelohe a hana paha e pili ana i ka hemoglobin synthesis, e hopena ana i nā hemoglobins ʻokoʻa a ʻokoʻa paha, e like me HbS, HbC, HbH a me Barts 'Hb.

No laila, mai ka hemoglobin electrophoresis, hiki ke hoʻomaopopo ʻia i ke alo o nā hemoglobins maʻamau a, me ke kōkua o kahi ʻano hana diagnostic i kapa ʻia ʻo HPLC, hiki ke nānā pono i ka paʻa o nā hemoglobins maʻamau a me nā ʻokoʻa, a he mau hōʻailona paha ia o:

I ke kumu o ke kuhi ʻia o ka anemia o ka cell sickle e ka test prick heel, ʻo ka hopena maʻamau ʻo HbFA (ʻo ia hoʻi, he HbA a me HbF ka pēpē, he mea maʻamau ia), ʻo ka hopena o ka HbFAS a me ka HbFS e hōʻike nei i ke ʻano o ka cell sickle. a me ka maʻi anemia ʻili maʻi.

Hiki ke hana ʻia ka ʻike ākea o ka thalassemias e nā hemoglobin electrophoresis e pili ana me HPLC, kahi e hōʻoia ʻia ai nā paʻa alpha, beta, delta a me gamma, e hōʻoiaʻiʻo ana i ka loaʻa ʻole a i ʻole ka loaʻa ʻana o kēia mau kaulahao globin a, e like me ka hopena. , e hoʻoholo i ke ʻano o ka thalassemia. E aʻo pehea e ʻike ai i ka thalassemia.

I mea e hōʻoia ai i ke kuhi o kēlā me kēia maʻi e pili ana i ka hemoglobin, pono e kauoha ʻia nā hoʻokolohua ʻē aʻe e like me ka hao, ferritin, nā dālā transferrin, me kahi helu piha o ke koko. E ʻike pehea e wehewehe ai i ka helu o ke koko.