Electrogoresis Hemoglobin

ʻO Hemoglobin kahi protein e lawe i ka oxygen i loko o ke koko. Ana nā electrogoresis hemoglobin i nā pae o nā ʻano ʻokoʻa o kēia protein i ke koko.

Pono kahi laʻana koko.

I ka hale hana, hoʻonoho ka ʻenehana i ka laʻana koko ma kahi pepa kūikawā a hoʻopili i kahi kaila uila. Neʻe nā hemoglobins ma ka pepa a hana i nā kāʻei e hōʻike ana i ka nui o kēlā me kēia ʻano hemoglobin.

ʻAʻole pono kahi hoʻomākaukau kūikawā no kēia hōʻike.

Ke hoʻokomo ʻia ke kui kele e huki i ke koko, manaʻo kekahi poʻe i ka ʻeha kaulike. ʻO ka manaʻo ʻē aʻe he prick a wīwī wale paha ka manaʻo. Ma hope, loaʻa paha i kahi kūpikipiki a i ʻole ka ʻūlū iki. Hele koke kēia.

Loaʻa paha iā ʻoe kēia hoʻokolohua inā manaʻo kāu mea lawelawe olakino loaʻa ʻoe i kahi maʻi i hoʻokumu ʻia e nā ʻano hemoglobin (hemoglobinopathy) maikaʻi ʻole.

Nui nā ʻano hemoglobin (Hb) like ʻole. ʻO nā mea maʻamau ʻo HbA, HbA2, HbE, HbF, HbS, HbC, HbH, a me HbM. ʻO nā mākua olakino nā pae koʻikoʻi o HbA a me HbA2 wale nō.

Loaʻa paha i kekahi poʻe ka liʻiliʻi o ka HbF. ʻO kēia ke ʻano nui o hemoglobin i ke kino o kahi pēpē i hānau ʻole ʻia. Pili kekahi mau maʻi me nā kiʻekiʻe HbF kiʻekiʻe (ke ʻoi aku ka nui o ka HbF ma mua o 2% o ka huina hemoglobin).

ʻO HbS kahi ʻano maʻamau o ka hemoglobin e pili ana i ka anemia maʻi maʻi. I ka poʻe me kēia ʻano, aia i nā hunaola ʻulaʻula i kekahi manawa ke ʻano crescent a i ʻole sickle. E wāwahi maʻalahi kēia mau pūnaewele a i ʻole hiki ke ālai i nā kīʻaha koko liʻiliʻi.

ʻO HbC kahi ʻano hemoglobin i pili ʻole me ka hemolytic anemia. ʻOi aku ka māmā o nā ʻōuli ma mua o ka maʻi maʻi anemia.

ʻO nā mea ʻē aʻe, ʻoi aku ka liʻiliʻi, nā mole Hb abnormal e hana i nā ʻano anemia like ʻole.

I ka poʻe mākua, he mau pākēneka maʻamau kēia o nā mole hemoglobin ʻokoʻa:

• HbA: 95% a 98% (0.95 a 0.98)
• HbA2: 2% a 3% (0,02 a 0,03)
• HbE: Loaʻa ʻole
• HbF: 0.8% a 2% (0.008 a 0.02)
• HbS: ʻAole
• HbC: ʻAole

I nā pēpē a me nā keiki, he pākēneka maʻamau kēia o nā mole HbF:

• HbF (hānau hou): 50% a 80% (0.5 a 0.8)
• HbF (6 mahina): 8%
• HbF (ma luna o 6 mau mahina): 1% a i 2%

Hiki ke loli iki nā pae waiwai maʻamau i waena o nā hale hana ʻokoʻa. Hoʻohana kekahi mau labs i nā ana like ʻole a i ʻole hoʻokolohua i nā laʻana like ʻole. E kamaʻilio i kāu mea hāʻawi e pili ana i ke ʻano o kāu mau hopena hōʻike.

Nā pae nui o nā hemoglobins pono ʻole e hōʻike:

• ʻO ka maʻi Hemoglobin C
• Hemoglobinopathy kakaikahi
• Anemia maʻi maʻi maʻi
• ʻO ka maʻi maʻi koko i hoʻoilina i ke kino i kahi ʻano hemoglobin (thalassemia)

Loaʻa paha iā ʻoe kahi hualoaʻa maʻamau a hewa ʻole paha inā ua loaʻa ʻoe i ke koko i loko o 12 mau pule o kēia hoʻokolohua.

Aia ka liʻiliʻi o ka makaʻu me ka lawe ʻia o kou koko. ʻOkoʻa nā aʻa a me nā aʻa i ka nui mai kekahi kanaka a i kekahi ʻē aʻe a mai kekahi ʻaoʻao o ke kino i kekahi. ʻOi aku ka paʻakikī o ka lawe ʻana i ke koko mai kekahi poʻe ma mua o nā poʻe ʻē aʻe.

ʻO nā pilikia ʻē aʻe e pili ana i ke kau ʻana o ke koko he liʻiliʻi akā hiki ke hoʻopili ʻia:

• Kahe nui ke koko
• Pāleʻale a ʻoluʻolu paha
• Hematoma (kūkulu koko ma lalo o ka ʻili)
• Maʻiʻaʻai (i iki kamepiula i kēlā me kēia manawa ka ʻili i haki)

Hb electrophoresis; Hgb electrophoresis; Electrophoresis - hemoglobin; Thallasemia - electrophoresis; Kele maʻi - electrophoresis; Hemoglobinopathy - electrophoresis

ʻO Calihan J. Hematology. I: Kleinman K, Mcdaniel L, Molloy M, eds. ʻO ka puke lima Harriet Lane. 22nd ʻepekema Piladelapia, PA: Elsevier; 2021: chap 14.

ʻO Elghetany MT, Schexneider KI, Banki K. Nā maʻi Erythrocytic. I: McPherson RA, Pincus MR, hua. ʻO kā Henry's Clinical Diagnosis a me Management e nā Laboratory Methods. 23rd Ed. St Louis, MO: Elsevier; 2017: chap 32.

ʻO RT. Hele i nā anemias. I: Goldman L, Schafer AI, eds. ʻO ka lāʻau lapaʻau Goldman-Cecil. 26th ed. Piladelapia, PA: Elsevier; 2020: chap 149.