ʻO Electrophoresis: he aha ia, he aha ia a pehea e hana ai

Anter

• No ke aha ia
• Pehea e hana ai
• Nā ʻano o electrophoresis
• 1. Hemoglobin electrophoresis
• 2. Palapala uila electrostoresis

ʻO Electrophoresis kahi hana ʻenehana i hana ʻia me ka pahuhopu o ka hoʻokaʻawale ʻana i nā mole e like me ko lākou nui a me ka uku uila i hiki ai ke ʻike ʻia nā maʻi, hiki ke hōʻoia ʻia ka ʻōlelo protein a i ʻole e ʻike ʻia nā microorganism

He hana maʻalahi a me ke kumu kūʻai haʻahaʻa ʻo Electrophoresis, hoʻohana ʻia i nā hana keʻena hoʻokolohua a me nā papahana noiʻi. Wahi a ke kumu o ka electrophoresis, pono paha e lawe i nā hoʻokolohua ʻē aʻe a me nā hoʻokolohua i mea e hiki ai i kahi hōʻailona, ​​e like paha

No ke aha ia

Hiki ke hana ʻia ʻo Electrophoresis no nā kumu he nui, i loko o nā papahana noiʻi a me ka hōʻoia, ʻoiai he hana maʻalahi a haʻahaʻa hoʻi ia.No laila, hiki ke hana ʻia nā electrophoresis i:

• Hoʻomaopopo i nā mea hoʻomaʻi, fungi, bacteria a me nā parasites, me ka hoʻohana mau ʻana o kēia noi i nā papahana noiʻi;
• Hōʻike makua;
• E hōʻoia i ka hōʻike o nā protein;
• ʻIke i nā hoʻololi, pono i ke kuhi o nā leukemias, no ka laʻana;
• E kālailai i nā ʻano o ka holo ʻana o ka hemoglobin, e pono ai i ke kuhi ʻana o ka maʻi anemia maʻi maʻi.
• Loiloi i ka nui o nā protein i loko o ke koko.

Wahi a ke kumu o ka electrophoresis, pono paha e lawe i nā hoʻokolohua kōkua ʻē aʻe no ke kauka e hoʻopau i ke kuhi.

Pehea e hana ai

E hana i ka electrophoresis pono ka gel, i hiki ke lilo i polyacrylamide a i ʻole agarose e like me ke kumu, electrophoresis buffer a me vat, mole mole mole a me ka pena wai fluorescent, i hoʻohui ʻia me ka UV a i ʻole nā ​​kukui uila LED, i ʻike ʻia he transilluminator .

Ma hope o ka hoʻomākaukau ʻana i ka gel, pono e hoʻokau i kahi mea kikoʻī e hana i nā luawai i loko o ka gel, i kapa nui ʻia ka waihona, a e hoʻonohonoho i ka gel. Ke mākaukau ka gel, hoʻopili wale i nā mea i nā luawai. No kēia, pono e kau i kahi māka mole mole i loko o kekahi o nā luawai, kahi mana maikaʻi, ʻo ia ka mea i ʻike ʻia he aha ia, kahi mana maikaʻi ʻole, ka mea e hōʻoia i ka pono o ka hopena, a me nā laʻana e nānā ʻia. Pono e kāwili ʻia i nā laʻana a pau me ka pena wai fluorescent, no ka mea hiki i kēia ala ke ʻike i nā kāʻei ma ka transilluminator.

Pono e hoʻokomo i ka gel me nā laʻana i loko o ka pou electrophoresis, kahi i loaʻa ka hopena buffer kikoʻī, a laila huli ʻia ka hāmeʻa a laila aia ka uila a, a no laila, he ʻokoʻa kūpono, a he mea nui ia no ka hoʻokaʻawale ʻana i nā ʻāpana e like me i kā lākou ukana a me ka nui. Hoʻololi ka manawa holo uila electroforetic e like me ke kumu o ke kaʻina hana, a hiki ke hala i 1 hola.

Ma hope o ka manawa i hoʻoholo ʻia, hiki ke ʻike i ka hopena o ka electrophoretic e holo i loko o ka transilluminator. Ke waiho ʻia ka gel ma lalo o UV a i ʻole ke kukui LED, hiki ke ʻike i ke kumu hoʻohālikelike: ʻo ka nui o ka mole, ʻo ka liʻiliʻi o ka neʻe ʻana, e hoʻokokoke ana i ka luawai, ʻoiai ka māmā o ka mole, ʻoi aku ka nui o ka hiki i ka neʻe ʻana.

No ka hōʻoia ʻana o ka hopena, pono pono ke ʻike ʻia nā kāʻei o ka mana maikaʻi a i ka kaohi maikaʻi ʻole ʻike ʻia kekahi mea, no ka mea inā ʻaʻole ia he hōʻailona aia kahi haumia, a pono e hana hou i ke kaʻina holoʻokoʻa.

Nā ʻano o electrophoresis

Hiki ke hana i nā uila no nā kumu like ʻole a, e like me ke kumu, hiki ke hoʻohana ʻia i kekahi ʻano ʻano gel, ʻo ka polyacrylamide a me ka agarose ka mea maʻamau.

ʻO ke electrophoresis e hoʻomaopopo ai i nā microorganism ʻoi aku ka maʻamau e hana ʻia i nā keʻena hoʻokolohua noiʻi, akā no nā hana diagnostic, hiki ke hoʻohana ʻia nā electrophoresis e ʻike i nā maʻi hematological a me nā maʻi e ulu me ka hoʻonui ʻana o ka nui o nā protein, ʻo ia nā ʻano nui o nā electrophoresis:

1. Hemoglobin electrophoresis

ʻO Hemoglobin electrophoresis kahi hana ʻenehana i hana ʻia e ʻike ai i nā ʻano hemoglobin e kahe ana i loko o ke koko, e hiki ai ke ʻike i ke kū ʻana o nā maʻi e pili ana i ka hemoglobin synthes. Hoʻomaopopo ʻia ke ʻano o ka hemoglobin e nā electrophoresis ma kahi kikoʻī PH, kūpono ma waena o 8.0 a me 9.0, me kahi ʻano o nā pēl e hōʻoia ʻia e hiki ke hoʻohālikelike ʻia i ke kumu maʻamau, e ʻae ana i ka ʻike o ke kūlike ʻole o nā hemoglobins.

He aha ka mea i hana ʻia no: Hana ʻia ʻo Hemoglobin electrophoresis e nānā a ʻike pono i nā maʻi e pili ana i ka hemoglobin synthesis, e like me ka anemia cell sickle a me ka maʻi hemoglobin C, a he mea pono no ka hoʻokaʻawale ʻana i ka thalassemia. E aʻo pehea e wehewehe ai i nā uila hemoglobin.

2. Palapala uila electrostoresis

ʻO ka protein electrophoresis kahi hōʻike i noi ʻia e ke kauka e nānā i ka nui o nā protein e holo ana i loko o ke koko a, no laila, e ʻike ai i nā maʻi. Hana ʻia kēia hoʻokolohua mai kahi hāpana koko, i centrifuged e loaʻa ai ka plasma, kahi ʻāpana o ke koko, ʻo ia hoʻi, i waena o nā mea ʻē aʻe, o nā protein.

Ma hope o electrophoresis, hiki ke ʻike ʻia kahi kumu o nā paʻa a, a laila, he pakuhi kahi e hōʻike ʻia ai ka nui o kēlā me kēia hapa o nā protein, ma ke ʻano he kumu no ka ʻike.

He aha ka mea i hana ʻia no: ʻO ka electrophoresis protein ka mea e ʻae ai i ke kauka e noiʻi i ka hanana o ka myeloma he nui, ka make wai, ka cirrhosis, ka mumū, ka maʻi ate, ka pancreatitis, ka lupus a me ke kiʻekiʻe ma muli o ke kaola band a me ka pakuhi i hōʻike ʻia i loko o ka hōʻike hōʻike.

Hoʻomaopopo pehea e hana ai a pehea e maopopo ai i ka hopena o ka protein electrophoresis.