Hōʻaʻo koko ELISA

Kū ʻo ELISA no immunoassay e pili ana i ka enzyme. Hoʻomaʻamaʻa pinepine ʻia ia e ʻike i nā mea ʻino i loko o ke koko. ʻO kahi antibody kahi protein i hana ʻia e ka ʻōnaehana pale o ke kino ke ʻike ʻo ia i nā mea ʻino, i kapa ʻia he antigens.

Pono kahi laʻana koko. ʻO ka hapanui o nā manawa, huki ʻia ke koko mai kahi aa i waiho ʻia ma loko o ka kuʻe lima a i ʻole ka hope o ka lima.

Hoʻouna ʻia kahi laʻana i kahi keʻena hoʻokolohua kahi e hoʻopili ʻia ai ka antibody i kuhi ʻia a i ʻole ka antigen i kahi ʻelima kikoʻī. Inā aia ka mea pahuhopu i loko o ka laʻana, huli ka hopena hōʻoia i kahi kala ʻokoʻa.

ʻAʻole pono kahi hoʻomākaukau kūikawā.

Ke hoʻokomo ʻia ke kui kele e huki i ke koko, manaʻo kekahi poʻe i ka ʻeha kaulike. ʻIke wale kekahi i kahi ʻōniʻu a i ʻole kui. Ma hope, loaʻa paha i kahi kūpikipiki a i ʻole ka ʻūlū iki. Hele koke kēia.

Hoʻohana pinepine ʻia kēia hoʻāʻo e ʻike inā ʻike ʻia ʻoe i nā maʻi ʻino a i ʻole nā ​​mea ʻē aʻe e hoʻopili ai i ka maʻi. Hoʻohana ʻia ia e pale no nā maʻi o kēia wā a i hala paha.

Aia nā waiwai maʻamau i ke ʻano o ka lāʻau i hōʻike ʻia. Hoʻohana kekahi mau keʻena hoʻokolohua i nā ana ʻokoʻa a hoʻāʻo paha i nā laʻana like ʻole. Hiki ke loli iki nā pae waiwai maʻamau i waena o nā hale hana ʻokoʻa. E kamaʻilio me kāu mea mālama ola e pili ana i ke ʻano o kāu mau hopena hōʻike kikoʻī.

Hilinaʻi nā waiwai maʻamau i ke ʻano o nā mea i hōʻike ʻia. I kekahi poʻe, he maʻamau ka hopena maikaʻi.

ʻAʻohe pilikia iki i ka lawe ʻia ʻana o kou koko. ʻOkoʻa nā aʻa a me nā aʻa i ka nui mai kekahi kanaka a i kekahi, a mai kekahi ʻaoʻao o ke kino i kekahi ʻē aʻe. ʻOi aku ka paʻakikī o ka lawe ʻana i ke koko mai kekahi poʻe ma mua o nā poʻe ʻē aʻe.

ʻO nā pilikia ʻē aʻe e pili ana i ke kau ʻana o ke koko he liʻiliʻi, akā hiki ke hoʻopili ʻia:

• Kahe nui ke koko
• Pāleʻale a ʻoluʻolu paha
• Nā punctures he nui e ʻimi i nā uaua
• Hematoma (hōʻiliʻili koko ma lalo o ka ʻili)
• Maʻiʻaʻai (i iki kamepiula i kēlā me kēia manawa ka ʻili i haki)

Immunoassay pili iā Enzyme; EIA

• Hōʻaʻo koko

Aoyagi K, Ashihara Y, Kasahara Y. Immunoassay a me immunochemistry. I: McPherson RA, Pincus MR, hua. ʻO kā Henry's Clinical Diagnosis a me Management e nā Laboratory Methods. 23rd Ed. St Louis, MO: Elsevier; 2017: chap 44.

ʻO Murray PR. Ke kauka lāʻau a me ka hale hōʻikeʻike microbiology. I: Bennett JE, Dolin R, Blaser MJ, hua pā. ʻO Mandell, Douglas, a me Bennett's Principal a me ka hoʻomaʻamaʻa ʻana i nā maʻi kūpikipiki, Hoʻopuka Hou. 8th ed. Piladelapia, PA: Elsevier Saunders; 2015: chap 16.